Rat Hepcidin (Hepc) ELISA Kit

HAMP ELISA Kit, HFE2B ELISA Kit, PLTR ELISA Kit, LEAP1 ELISA Kit, Hepcidin Antimicrobial Peptide ELISA Kit, Liver-expressed antimicrobial peptide 1 ELISA Kit, Putative liver tumor regressor ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Hepc. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hepc. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Hepc, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hepc in the samples is then determined by comparing the OD of the samples to the standard curve