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Superoxide Dismutases (SOD) Activity Colorimetric Assay Kit

Superoxide dismutase (SOD) Activity Assay Kit provides a colorimetric assay for the quantitative determination of SOD enzyme activity in biological fluids. In the assay, superoxide anion is provided by a xanthine oxidase (XO) catalyzed reaction.

Product Specifications

Field of Research

Metabolism Research

Sample Type

Serem, Plasma, Animal and Plant Tissues, Cells, Bacteria, Fungus

Storage Conditions

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Notes

For research use only.

Applications Notes

Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm.

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