General 1, 25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

DH-VD3 ELISA Kit, Calcitriol ELISA Kit, Rocaltrol ELISA Kit, Calcijex ELISA Kit, 1,25- (OH) 2D3 ELISA Kit, 1,25-Dihydroxycholecalciferol ELISA Kit, 1,25-Dihydroxy Vitamin D3 ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.