General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

5-Me-THFA ELISA Kit, 5-Me-H4FA ELISA Kit, 5-Me-THF ELISA Kit, 5-Me-H4F ELISA Kit, Levomefolic Acid ELISA Kit, Metafolin ELISA Kit, 5-Methyltetrahydrofolic Acid ELISA Kit, L-Methylfolate ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve.