Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit

MBL2 ELISA Kit, COLEC1 ELISA Kit, HSMBPC ELISA Kit, MBP1 ELISA Kit, MBP ELISA Kit, Collectin-1 ELISA Kit, Mannose-binding protein C ELISA Kit, Mannan Binding Protein ELISA Kit, Mannose-Binding Lectin (Protein C) 2, Soluble (Opsonic Defect) ELISA Kit

Standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve