Mouse Growth Arrest and DNA Damage Inducible Protein Gamma (GADD45g) ELISA Kit

CR6 ELISA Kit, DDIT2 ELISA Kit, GADD45γamma ELISA Kit, GRP17 ELISA Kit, Gadd-Related Protein,17 kD ELISA Kit, Cytokine-responsive protein CR6 ELISA Kit, DNA damage-inducible transcript 2 protein ELISA Kit

Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GADD45g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GADD45g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GADD45g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GADD45g in the samples is then determined by comparing the OD of the samples to the standard curve