Cattle Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

CXCL11 ELISA Kit, I-TAC ELISA Kit, IP9 ELISA Kit, SCYB11 ELISA Kit, SCYB9B ELISA Kit, B-R1 ELISA Kit, Chemokine (C-X-C-Motif) ligand 11 ELISA Kit, Small Inducible Cytokine Subfamily B Member 11 ELISA Kit, Interferon gamma-inducible protein 9 ELISA Kit

Standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ITaC in the samples is then determined by comparing the OD of the samples to the standard curve