Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

PLA2G1B ELISA Kit, PLA2A ELISA Kit, PPLA2 ELISA Kit, Phospholipase A2, Group IB ELISA Kit, Group IB phospholipase A2 ELISA Kit, Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig pPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig pPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig pPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig pPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve