General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

25-HVD3 ELISA Kit, 25HVD3 ELISA Kit, Calcifediol ELISA Kit, Calcidiol ELISA Kit, H-VD3 ELISA Kit, 25-Hydroxycholecalciferol ELISA Kit, 25-Hydroxy Vitamin D3 ELISA Kit, 25 (OH) D ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.