Mouse Growth Hormone (GH) ELISA Kit

GH1 ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, hGH-N ELISA Kit, Somatotropin ELISA Kit, Hygetropin ELISA Kit, Jintropin ELISA Kit, Kigtropin ELISA Kit, Pituitary Growth Hormone ELISA Kit, Growth Hormone, Normal ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GH in the samples is then determined by comparing the OD of the samples to the standard curve