Mouse Butyrylcholinesterase (BCHE) ELISA Kit

BuChE ELISA Kit, CHE1 ELISA Kit, E1 ELISA Kit, Pseudocholinesterase ELISA Kit, Cholinesterase ELISA Kit, Acylcholine acylhydrolase ELISA Kit, Butyrylcholine esterase ELISA Kit, Choline esterase II ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse BCHE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCHE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCHE in the samples is then determined by comparing the OD of the samples to the standard curve.