Rat Macrophage Inflammatory Protein 1 Gamma (MIP1g) ELISA Kit

CCL9 ELISA Kit, CCL10 ELISA Kit, SCYB9 ELISA Kit, CCF18 ELISA Kit, MRP2 ELISA Kit, C-C motif chemokine 9 ELISA Kit, Macrophage inflammatory protein-related protein 2 ELISA Kit, Small-inducible cytokine A9 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve