Rat Butyrylcholinesterase (BCHE) ELISA Kit

BuChE ELISA Kit, CHE1 ELISA Kit, E1 ELISA Kit, Pseudocholinesterase ELISA Kit, Cholinesterase ELISA Kit, Acylcholine acylhydrolase ELISA Kit, Butyrylcholine esterase ELISA Kit, Choline esterase II ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BCHE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BCHE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BCHE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BCHE in the samples is then determined by comparing the OD of the samples to the standard curve