Human Presenilin Associated Metalloprotease (PAMP) ELISA Kit

FTSH ELISA Kit, MEG4 ELISA Kit, YME1L1 ELISA Kit, YME1L ELISA Kit, ATP-dependent metalloprotease FtsH1 ELISA Kit, YME1 Like Protein 1 ELISA Kit, ATP-dependent zinc metalloprotease YME1L1 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAMP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAMP in the samples is then determined by comparing the OD of the samples to the standard curve