Rat FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) ELISA Kit

FRAP1 ELISA Kit, MTOR ELISA Kit, FRAP2 ELISA Kit, MTOR ELISA Kit, RAFT1 ELISA Kit, RAPT1 ELISA Kit, Mammalian Target Of Rapamycin ELISA Kit, FKBP12-Rapamycin Complex-Associated Protein 1 ELISA Kit, Rapamycin and FKBP12 target 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FRAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FRAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FRAP in the samples is then determined by comparing the OD of the samples to the standard curve