Mouse 17-Alpha-Hydroxylase (S17aH) ELISA Kit

CYP17A1 ELISA Kit, CPT7 ELISA Kit, P450C17 ELISA Kit, 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase ELISA Kit, Steroid 17-alpha-monooxygenase ELISA Kit, Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S17aH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S17aH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S17aH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S17aH in the samples is then determined by comparing the OD of the samples to the standard curve