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Human CASP8 and FADD Like Apoptosis Regulator (CFLAR) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8 And FADD Like Apoptosis Regulator (CFLAR) .

Product Specifications

Product Name Alternative

CASH ELISA Kit, CASP8AP1 ELISA Kit, CLARP ELISA Kit, Casper ELISA Kit, FLAME1 ELISA Kit, FLIP ELISA Kit, I-FLICE ELISA Kit, MRIT ELISA Kit, USURPIN ELISA Kit, c-FLIP ELISA Kit, c-FLIPL ELISA Kit, c-FLIPR ELISA Kit, c-FLIPS ELISA Kit, Caspase-eight-related protein ELISA Kit, FADD-like antiapoptotic molecule 1 ELISA Kit

UniProt

O15519

Reactivity

Human

Field of Research

Cell Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

0.056 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFLAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFLAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFLAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFLAR in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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