Human Interleukin 1 Theta (IL1q) ELISA Kit

IL1F10 ELISA Kit, IL38 ELISA Kit, FKSG75 ELISA Kit, IL-1HY2 ELISA Kit, IL-1F10 ELISA Kit, FIL1-Theta ELISA Kit, Interleukin-38 ELISA Kit, Interleukin-1 Receptor Antagonist FKSG75 ELISA Kit, Interleukin 1 Family, Member 10 ELISA Kit

Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1q. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1q. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1q, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1q in the samples is then determined by comparing the OD of the samples to the standard curve