Dog Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit

CD154 ELISA Kit, TRAP ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, TBAM ELISA Kit, T-BAM ELISA Kit, TNFSF5 ELISA Kit, Gp39 ELISA Kit, TNF Superfamily Member 5 ELISA Kit, Hyper-IgM Syndrome ELISA Kit, TNF-Related Activation Protein ELISA Kit, T-Cell B-Cell Activating Molecule ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CD40L in the samples is then determined by comparing the OD of the samples to the standard curve