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Human BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) .

Product Specifications

Product Name Alternative

C6.1A ELISA Kit, BRCC36 ELISA Kit, C6.1A ELISA Kit, CXorf53 ELISA Kit, Lys-63-specific deubiquitinase BRCC36 ELISA Kit, BRCA1-A complex subunit BRCC36 ELISA Kit, BRISC complex subunit BRCC36 ELISA Kit

UniProt

P46736

Reactivity

Human

Field of Research

Epigenetics & Chromatin

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.064 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRCC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRCC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRCC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRCC3 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

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