Human Egl Nine Homolog 1 (EGLN1) ELISA Kit

ECYT3 ELISA Kit, HIFPH2 ELISA Kit, PHD2 ELISA Kit, C1orf12 ELISA Kit, SM20 ELISA Kit, ZMYND6 ELISA Kit, HIF Prolyl Hydroxylase 2 ELISA Kit, Prolyl hydroxylase domain-containing protein 2 ELISA Kit, Hypoxia-inducible factor prolyl hydroxylase 2 ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGLN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGLN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGLN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGLN1 in the samples is then determined by comparing the OD of the samples to the standard curve