Mouse Neuronal PAS Domain Protein 4 (NPAS4) ELISA Kit

PASD10 ELISA Kit, NXF ELISA Kit, Le-PAS ELISA Kit, bHLHe79 ELISA Kit, Class E basic helix-loop-helix protein 79 ELISA Kit, HLH-PAS transcription factor NXF ELISA Kit, PAS domain-containing protein 10 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NPAS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPAS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NPAS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPAS4 in the samples is then determined by comparing the OD of the samples to the standard curve