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Mouse Toll Like Receptor Adaptor Molecule 1 (TICAM1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1) .

Product Specifications

Product Name Alternative

TRIF ELISA Kit, PRVTIRB ELISA Kit, Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta ELISA Kit, Proline-rich, vinculin and TIR domain-containing protein B ELISA Kit

UniProt

Q80UF7

Reactivity

Mouse

Field of Research

Infectious Disease & Virology; Immunology & Inflammation

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

22.8 pg/mL

Notes

For research use only.

Applications Notes

Standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TICAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TICAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TICAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TICAM1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

62.5-4000 pg/mL

Available Sizes

Frequently Asked Questions

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