Rat Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) ELISA Kit

CD256 ELISA Kit, APRIL ELISA Kit, TALL2 ELISA Kit, TRDL1 ELISA Kit, ZTNF2 ELISA Kit, A Proliferation Inducing Ligand ELISA Kit, TNF And APOL Related Leukocyte Expressed Ligand 2 ELISA Kit, TNF-Related Death Ligand 1 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFSF13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFSF13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFSF13 in the samples is then determined by comparing the OD of the samples to the standard curve