Rat Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit

HMFG ELISA Kit, MFGM ELISA Kit, SED1 ELISA Kit, BA46 ELISA Kit, EDIL1 ELISA Kit, OAcGD3S ELISA Kit, SPAG10 ELISA Kit, hP47 ELISA Kit, Medin ELISA Kit, Lactadherin ELISA Kit, Sperm Associated Antigen 10 ELISA Kit, Sperm Surface Protein hP47 ELISA Kit, Milk fat globule-EGF factor 8 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve