Rat Estrogen Related Receptor Alpha (ERRa) ELISA Kit

ERR1 ELISA Kit, ERRα ELISA Kit, ERRαlpha ELISA Kit, ESRL1 ELISA Kit, NR3B1 ELISA Kit, Estrogen receptor-like 1 ELISA Kit, Nuclear receptor subfamily 3 group B member 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ERRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ERRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ERRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ERRa in the samples is then determined by comparing the OD of the samples to the standard curve