Mouse Succinate Dehydrogenase Complex Subunit B (SDHB) ELISA Kit

SDH1 ELISA Kit, SDH ELISA Kit, Ip ELISA Kit, Iron Sulfur ELISA Kit, Iron-sulfur subunit of complex II ELISA Kit, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHB in the samples is then determined by comparing the OD of the samples to the standard curve