Rat Peroxiredoxin 1 (PRDX1) ELISA Kit

MSP23 ELISA Kit, NKEFA ELISA Kit, PAG ELISA Kit, PAGA ELISA Kit, PAGB ELISA Kit, TDPX2 ELISA Kit, Natural killer cell-enhancing factor A ELISA Kit, Proliferation-associated ELISA Kit, Thioredoxin peroxidase 2 ELISA Kit, Thioredoxin-dependent peroxide reductase 2 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRDX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRDX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRDX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRDX1 in the samples is then determined by comparing the OD of the samples to the standard curve