Rat Myosin Light Chain Kinase (MYLK) ELISA Kit

KRP ELISA Kit, MLCK108 ELISA Kit, MLCK210 ELISA Kit, MSTP083 ELISA Kit, MLCK ELISA Kit, smMLCK ELISA Kit, MYLK1 ELISA Kit, MLCK1 ELISA Kit, Telokin ELISA Kit, Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form ELISA Kit, Kinase-related protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYLK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYLK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYLK in the samples is then determined by comparing the OD of the samples to the standard curve