Human Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) ELISA Kit

TC-25 ELISA Kit, p21-Rac1 ELISA Kit, MIG5 ELISA Kit, Cell migration-inducing gene 5 protein ELISA Kit, Ras-like protein TC25 ELISA Kit, p21-Rac1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rac1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rac1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rac1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rac1 in the samples is then determined by comparing the OD of the samples to the standard curve