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Rat Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) .

Product Specifications

Product Name Alternative

APB-B3 ELISA Kit, SRA ELISA Kit, FE65L2 ELISA Kit, FE65-L2 ELISA Kit, Fe65L2 isoform 484 ELISA Kit, Alzheimer's beta-amyloid interaction protein ELISA Kit, Amyloid beta (A4) protein-binding, family B, member 3, isoform CRA_g ELISA Kit

UniProt

O35827

Reactivity

Rat

Field of Research

Neuroscience

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates and other biological fluids.

Sensitivity

0.062 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APBB3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APBB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APBB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APBB3 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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