Rat Mitogen Activated Protein Kinase 14 (MAPK14) ELISA Kit

CSBP1 ELISA Kit, CSBP2 ELISA Kit, CSPB1 ELISA Kit, EXIP ELISA Kit, Mxi2 ELISA Kit, PRKM14 ELISA Kit, PRKM15 ELISA Kit, RK ELISA Kit, SAPK2A ELISA Kit, P38 ELISA Kit, Mitogen-activated protein kinase p38 alpha ELISA Kit, Cytokine suppressive anti-inflammatory drug-binding protein ELISA Kit

Standard: 80 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAPK14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAPK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAPK14 in the samples is then determined by comparing the OD of the samples to the standard curve