Rat Farnesoid X Receptor (FXR) ELISA Kit

NR1H4 ELISA Kit, BAR ELISA Kit, FXR ELISA Kit, HRR1 ELISA Kit, RIP14 ELISA Kit, Nuclear Receptor Subfamily 1, Group H, Member 4 ELISA Kit, Bile acid receptor ELISA Kit, Farnesol receptor HRR-1 ELISA Kit, Retinoid X receptor-interacting protein 14 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FXR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FXR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FXR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FXR in the samples is then determined by comparing the OD of the samples to the standard curve