Rat Ghrelin (GHRL) ELISA Kit

MTLRP ELISA Kit, Preproghrelin ELISA Kit, Growth Hormone Secretagogue Receptor Ligand ELISA Kit, Appetite-regulating hormone ELISA Kit, Growth hormone-releasing peptide ELISA Kit, Motilin-related peptide ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.