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Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd) .

Product Specifications

Product Name Alternative

FAAR ELISA Kit, NR1C2 ELISA Kit, NUC1 ELISA Kit, NUCI ELISA Kit, PPARB ELISA Kit, PPARδ/B ELISA Kit, Nuclear Receptor Subfamily 1, Group C, Member 2 ELISA Kit, Nuclear hormone receptor 1 ELISA Kit, Peroxisome proliferator-activated receptor beta ELISA Kit

UniProt

P35396

Reactivity

Mouse

Field of Research

Cancer Biology, Metabolism Research, Signal Transduction

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.055 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPARd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPARd in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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