Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd) .
Product Specifications
Product Name Alternative
FAAR ELISA Kit, NR1C2 ELISA Kit, NUC1 ELISA Kit, NUCI ELISA Kit, PPARB ELISA Kit, PPARδ/B ELISA Kit, Nuclear Receptor Subfamily 1, Group C, Member 2 ELISA Kit, Nuclear hormone receptor 1 ELISA Kit, Peroxisome proliferator-activated receptor beta ELISA Kit
UniProt
P35396
Reactivity
Mouse
Field of Research
Cancer Biology, Metabolism Research, Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.055 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPARd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPARd in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL
Available Sizes
Frequently Asked Questions
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