Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit

FJHN ELISA Kit, TCF2 ELISA Kit, HNF1βeta ELISA Kit, HNF2 ELISA Kit, LFB3 ELISA Kit, LF-B3 ELISA Kit, MODY5 ELISA Kit, VHNF1 ELISA Kit, Transcription Factor 2, Hepatic ELISA Kit, Variant Hepatic Nuclear Factor ELISA Kit, Homeoprotein LFB3 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNF1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNF1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNF1b in the samples is then determined by comparing the OD of the samples to the standard curve