Human 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) ELISA Kit

HSD11β1L ELISA Kit, SCDR10 ELISA Kit, HSD3 ELISA Kit, SDR26C2 ELISA Kit, 11-Beta Hydroxysteroid Dehydrogenase Type 1 Like Protein ELISA Kit, Short chain dehydrogenase/reductase family 26C member 2 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSD11b3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSD11b3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSD11b3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSD11b3 in the samples is then determined by comparing the OD of the samples to the standard curve