Rat A Disintegrin and Metalloprotease 17 (ADAM17) ELISA Kit

CD156b ELISA Kit, TACE ELISA Kit, CSVP ELISA Kit, Tumor Necrosis Factor, Alpha, Converting Enzyme ELISA Kit, Disintegrin and metalloproteinase domain-containing protein 17 ELISA Kit, Snake venom-like protease ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADAM17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADAM17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADAM17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADAM17 in the samples is then determined by comparing the OD of the samples to the standard curve