Rat Pleiotrophin (PTN) ELISA Kit

HARP ELISA Kit, HBGF8 ELISA Kit, HBNF ELISA Kit, NEGF1 ELISA Kit, Heparin Binding Growth Factor 8 ELISA Kit, Neurite Growth-Promoting Factor 1 ELISA Kit, Heparin Affin Regulatory Peptide ELISA Kit, Heparin Binding Growth Associated Molecule ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTN in the samples is then determined by comparing the OD of the samples to the standard curve