Rat Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) ELISA Kit

CMD1S ELISA Kit, CMH1 ELISA Kit, MPD1 ELISA Kit, MYHCB ELISA Kit, Myopathy, Distal 1 ELISA Kit, Myosin heavy chain slow isoform ELISA Kit, Myosin heavy chain, cardiac muscle beta isoform ELISA Kit

Standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MYH7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MYH7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MYH7 in the samples is then determined by comparing the OD of the samples to the standard curve