Rat Coronin 1A (CORO1A) ELISA Kit

CLABP ELISA Kit, p57 ELISA Kit, CLIPINA ELISA Kit, HCORO1 ELISA Kit, TACO ELISA Kit, Coronin-1 ELISA Kit, Coronin, Actin-Binding Protein,1A ELISA Kit, Coronin-like protein A ELISA Kit, Coronin-like protein p57 ELISA Kit, Tryptophan aspartate-containing coat protein ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CORO1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CORO1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CORO1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CORO1A in the samples is then determined by comparing the OD of the samples to the standard curve