Rat Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit

PAK3 ELISA Kit, BETA-PIX ELISA Kit, COOL1 ELISA Kit, P50 ELISA Kit, P50BP ELISA Kit, P85 ELISA Kit, P85SPR ELISA Kit, PIXB ELISA Kit, SH3 Domain-Containing Proline-Rich Protein ELISA Kit, PAK-Interacting Exchange Factor Beta ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ARHGEF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ARHGEF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ARHGEF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ARHGEF7 in the samples is then determined by comparing the OD of the samples to the standard curve