Human Interferon Stimulated Gene 15 (ISG15) ELISA Kit

G1P2 ELISA Kit, IFI15 ELISA Kit, ISG15 ELISA Kit, Interferon Alpha-Inducible Protein clone IFI-15K ELISA Kit, Interferon Induced Protein 15 ELISA Kit, Interferon-induced 17 kDa protein ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ISG15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ISG15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ISG15 in the samples is then determined by comparing the OD of the samples to the standard curve