Cattle Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit

CXCL10 ELISA Kit, C7 ELISA Kit, IFI10 ELISA Kit, INP10 ELISA Kit, SCYB10 ELISA Kit, Crg2 ELISA Kit, GIP10 ELISA Kit, Mob1 ELISA Kit, Chemokine C-X-C Motif Ligand 10 ELISA Kit, Small Inducible Cytokine Subfamily B (Cys-X-Cys) Member 10 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IP10 in the samples is then determined by comparing the OD of the samples to the standard curve