Human Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) ELISA Kit

PS6K ELISA Kit, S6K ELISA Kit, S6K1 ELISA Kit, STK14A ELISA Kit, p70 (S6K) -alpha ELISA Kit, p70-S6K ELISA Kit, p70-alpha ELISA Kit, P70-S6 Kinase 1 ELISA Kit, 70 kDa ribosomal protein S6 kinase 1 ELISA Kit, Serine/threonine-protein kinase 14A ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RPS6Kb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RPS6Kb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RPS6Kb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RPS6Kb1 in the samples is then determined by comparing the OD of the samples to the standard curve