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Human MARCKS Related Protein (MARCKSL1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MARCKS Related Protein (MARCKSL1) .

Product Specifications

Product Name Alternative

MLP1 ELISA Kit, MRP ELISA Kit, MLP ELISA Kit, F52 ELISA Kit, MACMARCKS ELISA Kit, MARCKS-like protein 1 ELISA Kit, Macrophage myristoylated alanine-rich C kinase substrate ELISA Kit

UniProt

P49006

Reactivity

Human

Field of Research

Musculoskeletal & Connective Tissue Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.113 ng/mL

Notes

For research use only.

Applications Notes

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MARCKSL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MARCKSL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MARCKSL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MARCKSL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.32-20 ng/mL

Available Sizes

Frequently Asked Questions

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