Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit

ARP4 ELISA Kit, FIAF ELISA Kit, HFARP ELISA Kit, NL2 ELISA Kit, PGAR ELISA Kit, Pp1158 ELISA Kit, Fasting-Induced Adipose Factor ELISA Kit, Hepatic Angiopoietin-Related Protein ELISA Kit, Hepatic Fibrinogen/Angiopoietin-Related Protein ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANGPTL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANGPTL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANGPTL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANGPTL4 in the samples is then determined by comparing the OD of the samples to the standard curve