E2-230K/UBE2O Antibody
Goat polyclonal antibody to UBE2O
Product Specifications
Product Name Alternative
Anti UBE2O antibody, anti ubiquitin-conjugating enzyme E2O antibody, anti E2-230K antibody, anti FLJ12878 antibody, anti KIAA1734 antibody
Reactivity
Human
Target
E2-230K / UBE2O
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
ALLEAGMPECTEDK
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 4-6μg/ml
Molecular Weight
141
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/ml) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
63893
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