Rat Doublecortin (DCX) ELISA Kit

DBCN ELISA Kit, DC ELISA Kit, LISX ELISA Kit, SCLH ELISA Kit, XLIS ELISA Kit, Doublin ELISA Kit, Doublecortex ELISA Kit, Lissencephalin-X ELISA Kit, Lissencephaly, X-Linked ELISA Kit, Neuronal migration protein doublecortin ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DCX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DCX in the samples is then determined by comparing the OD of the samples to the standard curve