Rat Coagulation Factor XI (F11) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XI (F11) .
Product Specifications
Product Name Alternative
FXI ELISA Kit, PTA ELISA Kit, Fletcher Factor ELISA Kit, Prekallikrein ELISA Kit, Plasma Thromboplastin Antecedent ELISA Kit
Reactivity
Rat
Field of Research
Cardiovascular Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Plasma
Sensitivity
0.21 ng/mL
Notes
For research use only.
Applications Notes
Standard: 30 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F11 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.47-30 ng/mL
Available Sizes
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